Amplification

Life Sciences

Stratagene's expertise in enzyme engineering has translated into a broad portfolio of PCR enzymes and reverse transcriptases. Whether you need industry-leading accuracy, large product yields, or high sensitivity for cDNA synthesis, trust Strategene's high performance enzymes to address all of your amplification challenges.

Switch from Taq to Economic Paq5000™ DNA Polymerase today!

Stratagene's new Paq5000™ DNA Polymerase is an economical alternative to Taq that costs as little as five cents per unit. This new enzyme provides equivalent or better PCR yield than Taq DNA polymerase with reduced overall cycling time and can amplify up to 6-kb genomic targets.

Best in Class High Fidelity Pfu-based Fusion DNA Polymerases

Engineered for speed, Stratagene's new PfuUltra® II and Herculase® II Fusion DNA Polymerase, deliver superior yield and excellent reliability to help you amplify your productivity.

• Choose PfuUltra® II Fusion HS DNA Polymerase when industry-leading fidelity is critical
• Choose Herculase® II Fusion DNA Polymerase when superior yield and high fidelity are most important to you

Reverse Transcription at Your Preferred Temperature

Stratagene's AffinityScript™ Multiple Temperature Reverse Transcriptase delivers high cDNA yields whether priming at room temperature with random hexamers or at high, stringent temperatures for gene-specific primers or GC-rich sequences.

AccuScript™ High Fidelity Reverse Transcriptase Produces Nearly Error-free cDNA

Stratagene's new AccuScript™ high fidelity reverse transcriptase formulation delivers more than three-fold more accurate cDNA than any of the leading reverse transcriptases.

Lightning Fast Thermal Cycling

The 96-well fast and 384-well thermal blocks feature ramping speeds of up to     5°C/second and are optimized for fast PCR using Stratagene brand reagents, such as PfuUltra® II Fusion and Paq5000™ Hotstart DNA polymerases and fast site-directed mutagenesis using Stratagene brand QuickChange Lightning site-directed mutagenesis kits.

see PCR and RT-PCR Reagents brochure(1.37MB PDF) for a complete listing of Stratagene's PCR enzymes and the Stratagene Gradient Cycler Manual (849kB PDF) for complete instrument specifications.